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BIOCHEMISTRY OF ENZYME

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  • Department of Biochemistry
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Learning Outcomes

  • explain basic functions of enzymes
  • explain basic properties of enzymes
  • Defines the enzyme components
  • Express localization of enzymes in the cell
  • Defines the active site and catalytic activity of enzyme
  • explain working principle of enzymes
  • Express the relationship between enzyme and substrat
  • expalins activation energy of chemical reaction
  • will able to define the mechanisms of enzyme activity regulation
  • explains the definition of allosteric regulation
  • express regulation of enzymes by covalent modification
  • Express role of enzymatic activity in the regulation of protein synthesis
  • explain the factors that affect enzyme activity
  • explain the properties of enzyme-catalysed reactions
  • Discusses Michaelis-Menten kinetics
  • explain the Lineweaver-Burke graphic
  • define enzyme purification tecniques
  • defines chromatographic techniques
  • defines isoelectric point
  • discuss importance of enzymes in diagnosis of diseases
  • explain the importance of cardiac enzymes
  • defines enzymes associated with hepatic function
  • explain enzymes used in industry
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