PHARMACEUTICAL BIOTECHNOLOGY I

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Learning Outcomes

Will Be Able to List the History of Pharmaceutical Biotechnology and Explain Its Scope.
Comment on distinction and relationship between pharmaceuticals, biologicals and biopharmaceuticals.
Defines biotechnology.
Explains the uses of biotechnology in various fields (food, medicine, plant, environment and genetics).
Explains the relationship between biopharmaceuticals and pharmaceutical biotechnology.
Defines Pharmaceutical Biotechnology.
Will Be Able to Explain the Production of Antibiotics and Vitamins by Fermentation.
Defines penicillin biosynthesis.
Defines vitamin biosynthesis.
Will Be Able to Recognize Recombinant DNA and Explain the Methods Used in Its Production.
Lists the areas of use of recombinant DNA technology.
Explains the building blocks of nucleic acids.
Defines the vectors used in recombinant DNA technology.
Recognize plasmids and explain their production.
Defines restriction endonucleases and explains their functions.
Classifies host cells.
Defines and classifies DNA sequencing analyses.
Explains Polymerase Chain Reaction (PCR).
Explains the use of laboratory devices used in recombinant DNA analysis.
Will Be Able to Explain the Laboratory Techniques Used in Recombinant DNA Technology.
Defines the clean room.
Lists the devices in the clean room.
Lists the types of sterile cabinets and their working principles.
Classify the devices used for genetic material (DNA/RNA) analysis.
Classify the types of electrophoresis.
Explains the purpose of using the horizontal gel electrophoresis method.
Will Be Able to Explain Gene Cloning.
Explain the steps of gene cloning.
Lists eukaryotic and prokaryotic gene transfer methods.
Explains colony selection of vectors carrying recombinant genes.
Explains the control of the obtained recombinant gene product.
Will Be Able to Explain Gene Therapy and its Construction Methods
Defines gene therapy.
Defines Ex-vivo and In-vivo gene therapy.
Recognizes vectors used in gene therapy.
Lists viral and non-viral vectors.
Relates gene therapy and diseases.
Will Be Able to Explain Antisense Technology.
Define antisense technology.
Classify antisense oligonucleotides.
Explain the intracellular working mechanisms of antisense oligonucleotides.
Interpret the synthesis of antisense oligonucleotides according to the target gene.
Illustrate the loading methods of antisense oligonucleotides onto carrier systems.
Will Be Able to Explain Aptamers.
Define aptamer.
Lists the areas of use of aptamers.
Explains the therapeutic applications of aptamers.
Will Be Able to Explain the Structures of Therapeutic Proteins
Classify therapeutic proteins according to their biological functions.
Recognize the structure of the target protein.
Discusses the stability and folding of the target protein.
Discuss the post-translational modifications of the target protein.
Will Be Able to Discuss the Analysis Performed on Therapeutic Proteins.
Explains the vertical gel electrophoresis method.
Interpret vertical gel electrophoresis results.
List chromatographic analysis methods of therapeutic proteins.