PHARMACEUTICAL MICROBIOLOGY II

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Learning Outcomes

Will be able to list the isolation methods of microorganisms.
Explain the terms related to the isolation of microorganisms.
Lists the methods of detecting microorganisms.
Will be able to explain the detection methods of microorganisms.
List and explain biochemical diagnostic methods.
List and explain molecular diagnostic methods.
Will be able to define the measurement and counting methods of microbial growth.
Explains microbial growth curve and periods.
Explain how to prepare the reproductive curve, its importance and use.
Explains microbial counting methods.
Will be able to explain sterilisation.
Explains the definitions related to sterilisation.
List and explain sterilisation techniques.
Counts sterilisation control tests and explains their purposes.
Will be able to explain disinfection.
Explains disinfection techniques.
Lists disinfectants and explains their use.
Explain the mechanisms of action of disinfectants.
Explains the resistance to disinfectants.
Explains disinfectant efficacy detection tests.
Compare the differences between disinfection and sterilisation.
Explains the importance of hand hygiene.
Explain the microbiological control methods in the pharmaceutical production process.
Defines the pharmaceutical production process and evaluates microbiological aspects.
Explains good manufacturing practice and evaluates microbiological aspects.
Explains microbiological properties of pharmaceutical products.
Explains the definition of sterile, apyrogen product and bioburden.
Defines contamination sources.
Defines the types of water used.
Defines the properties of packaging materials.
Will be able to explain sterile working areas and working conditions.
Defines sterile working conditions.
Explains asepsis and aseptic zone.
Defines standardisation for sterile and non-sterile products.
Defines validation and explains validation processes. Evaluates microbiological aspects.
Çalışma alanlarındaki mikrobiyolojik yük sınıflandırmasını yapar.
Explain the properties and importance of pyrogen and endotoxin presence in sterile products.
Explains microbiological control methods in work areas.
Will be able to explain the control methods in sterile products.
Explains sterile pharmaceutical products and their properties.
Explains sterility control tests.
Will be able to explain the methods of control of microbial protective activity in drugs.
Defines the microbiological properties of parenteral and ocular drugs.
Explains microorganisms and microbial limits in other formulations.
Explains the standards of microbiological analysis method of drugs, counts reference microorganisms.
Lists the protective compounds found in products.
Counts and explains the protection control tests.
Will be able to explain microflora of foods and microbial spoilage.
Defines food hygiene.
Defines microbiological aspects of food production standards.
Classifies and defines microorganisms that can be found in foods and counts the factors of intoxication.
Defines microbial spoilage.
Classify and explain the factors affecting microbial growth in foods.
Defines food preservation protection methods.
Will be able to explain the determination of microorganism levels in foods.
Explains the detection methods of microorganisms in foods.
Defines hygiene and sanitation in food businesses.
List reference disinfectants.
Explain Microbiological Control Tests in Cosmetic Products.
Explains microbial hazards in cosmetic products.
Explains the sources of contamination in cosmetic products.
Defines the relevant microbial load in national and international regulations.
Explains microbial control tests in cosmetics.
Will be able to explain the processes of pharmaceutical products.
Defines the production process in industrial scale.
Makes the definition of continuous production systems bioreactor, explains the properties and purposes of use.
Explains industrial strain development studies.
List the microbial products produced industrially.
Defines bio-insecticide and explains with examples.
Explains bio-remediation.
Will be able to explain the processes of obtaining pharmaceutical products with genetic engineering techniques.
Defines gene engineering.
Explain molecular study techniques in microorganisms.
Examples of gene engineering applications, explains the products.
Explain the classification and general definitions of antimicrobial drugs.
Defines chemotherapeutic substance and explains chemotherapeutic substance sources.
Explains the discovery of chemotherapeutic agents.
Makes definitions related to antimicrobial activity.
List the microorganisms used for antibiotic production.
Explains the Mechanisms of Action of Antibiotics, Antifungals and Antivirals.
Explain the mechanisms of action of antibiotics on microorganisms (bacteria and fungi) with examples.
Explain antifungals and their mechanisms of action.
Explains antiviral drugs and their mechanisms of action.
Will be able to explain the mechanisms of microbial resistance.
Defines antimicrobial resistance and explains the mechanisms.
Counts the microorganisms causing superinfection and predisposing factors for infection.
Explains the development of single and multi-step resistance.
Explains the conscious use and importance of antibiotics.
Will be able to explain antibiotic susceptibility tests.
Defines antibiogram.
Classify and explain standard tests.
Classify the factors affecting antibiogram tests (medium, pH, humidity, atmosphere, temperature, inoculum, potency).
Counts reference test microorganisms (bacteria, yeast, mould).
Explains the theory and interpretation of the application of standard tests.
Explain macrobroth, microbroth, agar diffusion tests and methods.
Laboratory: Will be able to explain the rules to be considered in microbiological laboratory studies.
Lists the rules to be followed in the microbiology laboratory.
Explains aseptic techniques to work safely in the microbiology laboratory.
List the devices and equipment to be used in microbiological studies.
Explains the intended use of devices and equipment to be used in microbiological studies.
Laboratory: Explain microscopy.
Explains the types of microscopes and their intended use.
Explain the principles of use of microscopes.
Laboratory: Will be able to explain the equipment used in microorganism cultivation.
Sorts the equipment used for sowing.
Explains the intended use of this equipment.
Laboratory: Explain Glass Materials.
Lists the glass materials used in microbiology laboratory.
Explains the intended use of glass materials used in microbiology laboratory.
Laboratory: Will be able to explain sterilisation techniques.
Explains the concepts and application methods related to preparation
Lists and classifies microorganism staining methods.
Classifies the dyes used for microscopic examination and explains their properties.
Laboratory: Will be able to explain disinfection techniques.
Explains the concept of antisepsis.
Laboratory: Explain Sterilisation Control of Media Prepared in Petri Plates.
Laboratory: Explain hand disinfection.
Explains the purpose and application of contamination control of dirty, soap-washed, zefiran-applied hands.
Laboratory: Explain the preparation of the medium.
Defines the concept of pasteurisation, explains the areas of use.
Explains how to control autoclave sterilisation through tape
Explains how to control the sterilised media by incubation in the oven
Lists the stages of medium preparation and explains the application stages.
Laboratory: Explain the preparation of solid and liquid media.
Explains the devices used to prepare the medium and the stages and purposes of use.
Explain the differences between solid and liquid media.
List the differences in the stages of solid and liquid media preparation.
Explains how the distribution of media will be carried out.
Explains how to perform sterilisation of media.
Laboratory Explain sterilisation practices.
Explain the concepts of sterilisation and disinfection.
Explain the difference between chemical and physical sterilisation.
List the devices used.
List the chemicals used.
Laboratory: Explain the application of different sowing techniques.
Lists and explains the concepts related to microbial cultivation techniques.
Explains and lists the sowing rules and stages.
Explains the differences between sowing on different media.
Explain the applications of different cultivation techniques.
Laboratory: Explain the application of examination techniques in solid and liquid culture.
Explain the colony forms of prokaryotic organisms cultured in petri dish.
Explain microbial growth in liquid media.
Explain the morphology of yeasts in solid and liquid media.
Describe different pigmented colonies.
Explain the morphology of different organisms on different media
Describe the morphology of the same organism in different media.
Laboratory: Explain the preparation and examination of bacterial preparation.
Explains the concepts and application methods related to preparation
List and classify microorganism staining methods.
Classifies the dyes used for microscopic examination and explains their properties.
Explains the simple dyeing method and application methods
Explains Gram staining method and application methods
Laboratory: Explain microscopic examination of mould and yeast.
Explains how to prepare a mould preparation
Explains the staining methods and application methods for microscopic examination of moulds.
Explains the staining methods and application methods for microscopic examination of yeasts
Explains the capsule staining method.
Explains the spore staining method.
Laboratory: Will be able to explain DNA Isolation.
Explain the steps of DNA isolation.
Explain the mechanism of DNA isolation from microorganisms by phenol-chloroform technique.
Laboratory: Will be able to explain carbohydrate metabolism.
List and explain the media to be used in carbohydrate fermentation test.
Describes the microbial development in media containing monosaccharides, disaccharides and polysaccharides, microbial metabolism in these media and explains the products formed.
Explains how to make microbial classification by interpreting the test.
Laboratory: Explain Protein Metabolism.
List and explain the media to be used in protein metabolism tests.
Explain how different protein sources are metabolised.
Explains how to make microbial classification by interpreting the test.
Laboratory:Will be able to explain the open Petri method.
Defines the pharmaceutical industry and microbiological working conditions.
Laboratory:Will be able to explain the swap method.
Defines microbiological control of ambient air by open petri method and explains the application method.
Explains how to take samples from the counters by swap method and cultivation and examination of the culture by single colony method in blood and nutrient agar
Laboratory: Explain the microbiological analysis of lotion.
Defines and classifies microbiological risks in cosmetic products.
Defines the microorganisms in the cosmetic product sample.
Laboratory: Explain Antimicrobial Substances and Their Activities.
Explain the substances showing antimicrobial activity.